Table 2. Antibodies used for immunofluorescence of the ileum.

Antibody	Cell population	Species	Dilution	Company	Catalogue number
Primary KI67/MKI67	Proliferating cells	Rabbit	1:500	Abcam (Cambridge, UK)	Ab15580
Primary KI67	Proliferating cells	Rat	1:500	Thermo Fisher Scientific (Waltham, MA, USA)	14-5698-82
Primary OLFM4	Intestinal stem cells	Rabbit	1:500	Cell Signalling Technology (Danvers, MS, USA)	39141
Primary CC3	Apoptosis	Rabbit	1:200	Cell Signalling Technology (Danvers, MS, USA)	9661
Primary MPO	Neutrophils	Mouse	1:200	R&D systems (Minneapolis, MN, USA)	MAB3174
Secondary Anti-rabbit IgG a	Green fluorescence (488 nanometer)	Donkey	1:500	Cell Signalling Technology (Danvers, MS, USA)	1834802
Secondary Anti-mouse IgG a	Green fluorescence (488 nanometer)	Goat	1:1000	Cell Signalling Technology (Danvers, MS, USA)	4408
Secondary Anti-rat IgG a	Green fluorescence (488 nanometer)	Goat	1:1000	Cell Signalling Technology (Danvers, MS, USA)	4416
Secondary Anti-rabbit IgG a	Red fluorescence (568 nanometer)	Goat	1:1000	Thermo Fisher Scientific (Waltham, MA, USA)	A11036

^aImmunoglobulin G.