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. 2021 Dec 2;16(12):e0259130. doi: 10.1371/journal.pone.0259130

Fig 4. Reductions in the levels of catabolic markers and inflammatory cytokines in OA rats and human chondrocytes.

Fig 4

(A, B) Immunohistochemical staining was used to identify MMP1 and MMP3. (C) qRT-PCR analysis of the levels of mRNAs encoding MMP3, MMP13 and MCP-1 in IL-1β (20 ng/mL)-stimulated OA chondrocytes in the presence or absence of (various concentrations of) GLME (10, 100, 250 μg/mL) and Celecoxib (10 μM). (D) IL-6 and MCP-1 levels in cell supernatants were measured via sandwich ELISAs. (E) OA chondrocyte cell viability in the presence or absence of GLME, as revealed by the CCK8 assay. *P < 0.05, **P < 0.01, and ***P < 0.001 compared to the vehicle-treated group.