Table 2. IGF2 mRNA expression by RT-qPCR.
| Days after SNI | IGF2 mRNA expression (fold of sham) | P-value | |||
|---|---|---|---|---|---|
| Sham | SNI | SNI + siRNA IGF2 | 1 | 2 | |
| D2 | 1 | 28.7±6.0 | 2.5±3.2 | <0.001 | <0.001 |
| D3 | 1 | 1.0±1.0 | 0 | 0.988 | 0.421 |
| D5 | 1 | 1.1±0.6 | 0.5±0.1 | 1.0 | 0.134 |
| D7 | 1 | 1.4±0.9 | 0.2±0.1 | 0.562 | 0.293 |
Values are mean ± standard deviation; n = 4; P-value 1: sham group compared with SNI group; P-value 2: SNI + siRNA IGF2 group compared with SNI group. P < 0.05: statistically significant. SNI, spared nerve injury; siRNA, small-interfering RNA; IGF2, insulin-like growth factor-2; BL, baseline; D, day; RT-qPCR, real-time quantitative polymerase chain reaction.