. 2021 Dec 2;16(12):e0260765. doi: 10.1371/journal.pone.0260765

Table 2. ANOVA models describing treatment and experiment effects of log-normal distributed quantified liver metabolites values.

Metabolite		Treatment						Experiment Ln(normalised conc.)
		Ln(normalised conc.)						Experiment Ln(normalised conc.)
		NC	HFD	HFD+B420	HFD+PDX	HFD+B420+PDX	P	I	II	P
SCFA & Alcohols	Acetate	-4.79^a	-5.83^b	-5.63^b	-4.73^a	-4.87^a	<0.0001	-4.90	-5.45	<0.0001
	Ethanol	-2.74^a	-3.75^b	-3.71^b	-2.82^a	-3.00^a	0.001	-2.66	-3.75	<0.0001
	Glycerol	-4.24^a	-4.74^b	-4.85^b	-4.04^a	-3.76^a	<0.0001	-4.07	-4.58	0.004
Amino Acids	Alanine	-4.83^b	-5.36^c	-5.44^c	-4.28^a	-4.71^b	<0.0001	-4.75	-5.09	0.003
	Glycine	-4.29^a	-4.93^b	-4.89^b	-4.03^a	-4.25^a	<0.0001	-4.35	-4.61	0.06
	Glutamine	-3.67^ab	-4.20^c	-3.96^bc	-3.38^a	-3.56^ab	0.0001	-3.66	-3.85	0.09
	Isoleucine	-5.24	-5.92	-5.94	-5.56	-5.75	0.58	-5.53	-5.83	0.13
	Leucine	-3.68	-4.17	-4.00	-3.82	-3.56	0.11	-3.84	-3.85	0.68
	Valine	-4.67^a	-5.82^b	-6.11^b	-5.13^a	-5.04^a	<0.0001	-5.08	-5.63	0.002
Metabolism & Others	Ascorbate	-4.69^ab	-4.89^b	-4.84^b	-4.29^a	-4.58^ab	0.029	-4.63	-4.69	0.57
	Betaine	-4.59^bc	-4.85^cd	-5.07^d	-4.09^a	-4.31^ab	<0.0001	-4.30	-4.86	<0.0001
	Choline	-4.75^a	-5.24^b	-5.35^b	-4.42^a	-4.40^a	<0.0001	-4.45	-5.21	<0.0001
	Creatine	-6.16^ab	-7.09^c	-6.98^c	-5.95^a	-6.48^b	<0.0001	-6.29	-6.77	0.008
	Glucose	-0.48^a	-0.88^b	-0.91^b	-0.29^a	-0.47^a	0.0008	-0.30	-0.92	<0.0001
	Glutathione	-4.01^b	-4.47^c	-4.37^bc	-3.75^a	-3.90^a	0.001	-4.21	-3.99	0.18
	Glycerophosphocholine	-2.48^b	-2.59^b	-2.58^b	-1.76^a	-2.05^a	<0.0001	-2.24	-2.35	0.25
	Glycogen	2.52^a	2.11^b	1.90^b	2.57^a	2.66^a	0.046	-1.38	-1.78	0.07
	Lactate	-3.70^a	-4.32^b	-4.21^b	-3.56^a	-3.75^a	0.0002	-3.72	-4.09	0.006
	Taurine	-1.57	-1.78	-1.75	-1.33	-1.50	0.089	-1.38	-1.79	0.002
	Lipid	1.68^c	2.28^ab	2.42^a	1.72^c	2.02^b	<0.0001	1.89	2.16	0.004

Least-square mean values within a row with unlike superscript letters were significantly different for the treatment or experiment effects (P<0.05).