FIG. 2.

Amplification of the 209- and 191-bp fragments. Lane M, HaeIII-digested φX174 replicative-form DNA. (A) PCR with BV, HSV-1, or HSV-2 DNA as the template. Lanes 1 to 3, BV (strain E-2490) DNA as the template; lanes 4 to 6, HSV-1 (strain HF); lanes 7 to 9, HSV-2 (strain UW268). Lanes 1, 4, and 7, PCR with primers gGS4 and gGAS4; lanes 2, 5, and 8, PCR with primers gGS5 and gGAS5; lanes 3, 6, and 9, PCR with primers BV1 and BV2 according to the modified method of Scinicariello et al. (9, 10). Note that 128-bp products were obtained with BV and HSVs as the DNA templates. (B) PCR in the presence of contaminating viral DNA. Lanes 1 and 4, BV DNA alone as the template; lanes 2 and 5, BV DNA plus HSV-1 and HSV-2 DNA (800 TCID₅₀ each); lanes 3 and 6, BV DNA plus 1,000 copies of hepatitis B virus genomic DNA. Lanes 1 to 3, PCR with primers gGS4 and gGAS4; lanes 4 to 6, PCR with primers gGS5 and gGAS5.