FIG. 3.

PCR with serially diluted BV DNA as the template. Lanes 1, 4, and 7, nondiluted BV DNA; lanes 2, 5, and 8, 10-fold dilution; lanes 3, 6, and 9, 100-fold dilution. The three triangles indicate that the concentration of BV DNA decreased from lanes 1 to 3, from lanes 4 to 6, and from lanes 7 to 9. Lane M, HaeIII-digested φX174 replicative-form DNA. Lanes 1 to 3, PCR product obtained with primers gGS4 and gGAS4; lanes 4 to 6, PCR product obtained with primers gGS5 and gGAS5; lanes 7 to 9, PCR product obtained with primers BV1 and BV2 according to the modified method of Scinicariello et al. (9, 10).