| Strain, strain background (Escherichia coli) |
DH5a |
NEB |
Cat# 2987H |
Chemically competent cells |
| Strain, strain background (Escherichia coli) |
BL21(DE3) |
Novagen |
Cat# 71400 |
Chemically competent cells |
| Cell line (Homo sapiens) |
MCF7 |
Joan Brugge, Harvard |
|
|
| Cell line (M. musculus)
|
MVD7
|
Bear et al., 2000
|
|
|
| Cell line (Homo sapiens) |
HEK293T |
ATCC |
|
|
| Antibody |
anti-ENAH (rabbit polyclonal) |
Sigma |
Cat# HPA028448 |
(1:100) |
| Antibody |
anti-Paxillin (mouse monoclonal) |
BD Biosciences |
Cat# 612405 |
(1:200) |
| Antibody |
Goat anti-mouse Alexa Fluor 488 (goat polyclonal) |
Thermo Fisher |
|
(1:1000) |
| Antibody |
Goat anti-mouse Alexa Fluor 647 (goat polyclonal) |
Thermo Fisher |
|
(1:1000) |
| Antibody |
Donkey anti-rabbit Alexa Fluor 405 (donkey polyclonal) |
Abcam |
|
(1:1000) |
| Antibody |
SureLight Allophycocyanin-anti-FLAG antibody (mouse monoclonal) |
Perkin Elmer |
Cat# AD0059F |
(1:100) |
| Antibody |
anti-actin (mouse monoclonal) |
ProteinTech Group |
|
(1:2500) |
| Antibody |
anti-GAPDH (mouse monoclonal) |
Cell Signaling Technology |
Cat# 5174S |
(1:000) |
| Antibody |
anti-EVL (rabbit polyclonal) |
Sigma |
Cat# HPA018849 |
(1:1000) |
| Antibody |
anti-VASP (rabbit monoclonal) |
Cell Signaling Technology |
Cat# 3132S |
(1:1000) |
| Peptide, recombinant protein |
Streptavidin, R-Phycoerythrin Conjugate (SAPE) |
Thermo Fisher |
Cat# S866 |
(1:100) |
| Chemical compound, drug |
Alexa Fluor 488-Phalloidin |
Thermo Fisher |
|
(1:40) |
| Chemical compound, drug |
Alexa Fluor 647-Phalloidin |
Thermo Fisher |
|
(1:40) |
| Recombinant DNA reagent |
pLKO.1-Enah shRNA (plasmid) |
GE Dharmacon |
|
|
| Recombinant DNA reagent |
pLKO.1-Evl shRNA (plasmid) |
Sigma |
|
|
