| Strain, strain background (Rattus norvegicus, females) |
Sprague-Dawley rats |
Envigo |
#002 (RRID:MGI:5651135) |
200–220 g, approximately 10–12 weeks of age |
| Antibody |
Anti-GFP(rabbit polyclonal) |
abcam |
ab290 (RRID:AB_303395) |
IHC (1:5,000) |
| Antibody |
Anti-VGAT (goat polyclonal) |
Frontier Institute |
VGAT-Go-Af620 (RRID:AB_2571623) |
IHC (1:100) |
| Antibody |
Anti-VGlut1 (guinea pig polyclonal) |
Synaptic Systems |
135–304 (RRID:AB_887878) |
IHC (1:500) |
| Antibody |
Anti-VGlut2 (guinea pig polyclonal) |
Synaptic Systems |
135–404 (RRID:AB_887884) |
IHC (1:250) |
| Antibody |
Anti-synaptophysin (guinea pig polyclonal) |
Synaptic Systems |
101–004 (RRID:AB_1210382) |
IHC (1:750) |
| Other |
Neurotrace 640/660 |
Fisher Scientific |
2047616(RRID:AB_2572212) |
IHC (1:50)Stain selective for Nissl substance. |
| Antibody |
Anti-rabbit Alexa-Fluor Plus 488 (donkey polyclonal) |
Invitrogen |
A32790(RRID:AB_2762833) |
IHC (1:400) |
| Antibody |
Anti-guinea pig Alexa-Fluor 594 (donkey polyclonal) |
Jackson ImmunoResearch |
#706-585-148 (RRID:AB_2340474) |
IHC (1:200) |
| Antibody |
Donkey anti-goat Alexa-Fluor 647 (donkey polyclonal) |
Jackson ImmunoResearch |
#705-605-147 (RRID:AB_2340437) |
IHC (1:200) |
| Genetic reagent (Rattus norvegicus, females) |
HiRet-TRE-EGFP.eTeNT |
Generous gift from Tadashi Isa |
n/a |
Intraspinal injections of 1.6 × 107 vp/ml |
| Genetic reagent (Rattus norvegicus, females) |
AAV2-CMV-rtTAV16 |
Generous gift from Tadashi Isa |
n/a |
Intraspinal injections of 4.8 × 1012 vp/ml |
| Genetic reagent (Rattus norvegicus, females) |
HiRet-Cre |
Generous gift from Zhigang He |
n/a |
Intraspinal injections of 1.6 × 1012 vp/ml |
| Genetic reagent (Rattus norvegicus, females) |
AAV2-CAG-FLEx-GFP |
UNC Vector Core |
Ed Boyden 100 μl – Control Vectors |
Intraspinal injections of 3.5 × 1012 vp/ml |
| Chemical compound, drug |
Non-immune sera (rabbit IgG) |
Jackson ImmunoResearch |
#711-005-152 (RRID:AB_2340585) |
IHC (1:5,000) |
| Chemical compound, drug |
Sylgard |
Dow Corning |
Sylgard 184 Silicone Elastomer Kit |
Applied to walkway chamber |
| Software, algorithm |
SPSS |
IBM |
RRID:SCR_002865
|
Version 22 |
| Software, algorithm |
SigmaPlot |
Systat |
RRID:SCR_003210
|
Version 11, used to generate polar plots |
| Software, algorithm |
MaxTRAQ |
Innovision Systems |
n/a |
Motion capture analysis |
| Software, algorithm |
Matlab |
Mathworks |
|
Custom-written code to analyze laminar distribution |
| Software, algorithm |
Excel |
Microsoft |
|
Custom-written code to analyze gait |
