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. 2021 Dec 3;9(5):1290–1300. doi: 10.1016/j.gendis.2021.11.007

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 5 — Detection of neutralizing antibodies against D614G, B.1.1.7 and B.1.617 pseudotyped viruses in CoronaVac vaccinee serum samples. (A) Neutralizing activity of CoronaVac (inactivated vaccine) sera (n = 20) to D614G, B.1.1.7 and B.1.617. Pseudotypes were incubated with different serum dilutions for 60 min at 37 °C, and then were incubated onto 293T-ACE2 cells. Upon 72 h, cells were lysed with passive lysis buffer and the activity of firefly luciferase was analyzed. The half-maximal neutralizing titer (ID₅₀) was quantitatively analyzed using Graphpad 8.0 and the threshold of ID₅₀ was 1:40. (B) The GMT values and changes in CoronaVac vaccinee sera ID₅₀ against D614G, B.1.1.7 and B.1.617.