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. 2021 Oct 18;118(43):e2115430118. doi: 10.1073/pnas.2115430118

Fig. 4.

Fig. 4.

The bulk of SP-Pck1 degradation requires Icp55 aminopeptidase, and the effect of overexpressed Fra1 aminopeptidase on the degradation of APPP-Aro10. (A) Lane 1, kilodalton markers. Lanes 2 to 5, Tc/PRT chase of SP-Pck13f in wild-type (wt) cells. Lanes 6 to 9, the same as lanes 2 to 5 but in gid4Δ cells. Lanes 10 to 13, the same as lanes 2 to 5 but with SS-Pck13f. Lanes 14 to 17, the same as lanes 2 to 5 but in icp55Δ cells. (B) Quantification of data in A. (C) Lane 1, kilodalton markers. Lanes 2 to 5, Tc/PRT chase of APPP-Aro103f in wt cells. Lanes 6 to 9, the same as lanes 2 to 5 but in wt cells overexpressing Fra1myc from the PCUP1 promoter on a high-copy plasmid. Lanes 10 to 13, the same as lanes 2 to 5 but in gid2Δ cells lacking the active GID Ub ligase. (D) Quantification of data in C. Regarding the absence of SD bars in B and D: a detailed explanation is in the text and in Fig. 1. Blue stars on the left denote kilodalton markers.