Fig. 4.

Gα₁₃ signaling promotes Tfh cell differentiation in a cell-intrinsic manner. (A–C) Mixed bone marrow (BM) chimera experiments were conducted according to the scheme shown in A. Inguinal LNs were analyzed on day 9. (B) Summary of respective donor Tfh and GC-Tfh frequencies (n = 5 per group). (C) gMFI values of Tfh marker and molecules denoted as arbitrary units (A.U.) (n = 6 per group). (D–F) OT-II cotransfer experiment was conducted according to the scheme shown in D. Inguinal LNs were analyzed on days 0, 3, 5, and 7. (E) Summary of respective donor Tfh and GC-Tfh frequencies. (F) gMFI values of Tfh marker and molecules (n = 5 per group). (G) Experimental scheme of conjugate formation assay. (H) Antigen-specific T-DC and T-B conjugate efficiencies of WT or Gα₁₃-deficient OT-II Tfh cells (n = 3 per group). All data are representative of two independent experiments. All data are mean ± SEM; ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001; and ****P < 0.0001.