Fig. 2.

NE promotes an early transient 5-HT release. (A) A single PG from a rat was sliced into three to four slices (400 μm thick), and each slice was further sliced into three to five strips (300 to 400 μm thick). (B) Schematic drawing of a single pineal strip in one well of a 96-well plate. Individual strips were incubated in M199 for 30 min and then in M199 (Control) or M199 plus 1 μM NE or 50 μM ACh for another 40 min. After each incubation, the medium was collected and used to measure 5-HT (C), NAS (D), and melatonin (E) levels by UPLC/MS analysis. Fold increases are given as test period divided by baseline period and compared using a t test. **P < 0.01. Results are presented as mean ± SEM.