Fig. 4.

5-HT is released from pineal strips via a decynium-sensitive plasma membrane monoamine transporter. (A) Schematic of a single strip inserted into a microchamber connected to a peristaltic pump for continuous perifusion. (B) Time course of 5-HT secretion during drug exposure from UPLC/MS analysis of 20-min samples of the solution passing through the pineal strip. Open circles are control and closed circles are the secretion in the presence of 50 μM decynium-22, a selective inhibitor of equilibrative cation transporters, applied only during drug periods D1, D2, and D3.