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. 2021 Oct 21;118(43):e2113852118. doi: 10.1073/pnas.2113852118

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Fig. 5. — 5-HT does not change cAMP levels in isolated pinealocytes. The cAMP levels were analyzed by FRET. (A) A schematic diagram of the cAMP biosensor, Epac1, sandwiched between CFP (donor) and YFP (acceptor). Pinealocytes were transfected with the Epac1 probe by magnetofection. Once cAMP binds to Epac1, the conformational change increases the distance between CFP and YFP and thereby decreases FRET efficiency. (B) Forskolin, increasing cAMP, lowers FRET efficiency in pinealocytes. (C) NE activates endogenous β-adrenergic receptors and increases cAMP levels in pinealocytes. Neither 10 μM 5-HT (D) nor 30 μM 5-HT (E) affected the level of cAMP in pinealocytes.