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5-HT does not activate an ionotropic 5-HT3 receptor in pinealocytes. Representative recordings of 5-HT– and ACh-induced currents from a pinealocyte in whole-cell patch-clamp recording. Six of six cells showed no current with 5-HT and all of them exhibited significant inward currents with ACh. Holding potential was −60 mV. ACh (50 μM) and 5-HT (30 μM) were applied to the cell during the bar using local perfusion.
