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View full-text article in PMC

. 2021 Oct 21;118(43):e2113852118. doi: 10.1073/pnas.2113852118

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Fig. 7. — 5-HT activates 5-HT_2C receptors in the membrane of pinealocytes and raises calcium. Intracellular calcium levels were monitored by calcium imaging using Fura-2 AM fluorescence ratios (340/380 nm). (A) Calcium time course during application of 5-HT and NE. (B) Calcium time course in pinealocytes preincubated for 10 min with 0.5 μM RS, a selective 5-HT_2C antagonist, and then measured during application of 5-HT with RS and NE. (C) Magnified traces from the red-dashed box (A and B), showing that RS blocked the induction of a modest calcium increase by 5-HT. Closed circles indicate the application of 5-HT (A) and open circles indicate coapplication of 5-HT and RS (B). (D) Like 5-HT, WAY, a potent and selective 5-HT_2C agonist, increased intracellular calcium by a pathway blocked by RS.