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. 2021 Oct 20;118(43):e2110934118. doi: 10.1073/pnas.2110934118

Fig. 3.

Fig. 3.

ZsGreen-positive cells in the cuneiform nucleus express the vesicular glutamatergic transporter 2 (Vglut2) mRNA in Vglut2-ZsGreen mice. (A) Coronal brainstem slices from a Vglut2-ZsGreen mouse at the level of the cuneiform nucleus, showing the mRNAs of ZsGreen (green), Vglut2 (white) revealed by RNAscope experiments, and a coloration of the nuclear marker DAPI (blue). (B–D) Magnification of the slice in A, showing the location of the two markers in the cuneiform nucleus. (E–G) Magnification of the yellow squares in B–D, showing many examples of cells coexpressing Vglut2 and ZsGreen mRNAs (white arrows) in the cuneiform nucleus. CnF, cuneiform nucleus; IC, inferior colliculus; PAG, periaqueductal gray.