Figure 4.
mRNA-1273 elicits memory B cells that bind both WA1 and B.1.617.2
(A) Representative flow cytometry plots showing WA1 S-2P-specific, B.1.617.2 S-2P-specific, and dual-binding memory B cells at weeks 6, 25, and 42 post-immunization and day 28 post-challenge. Top row, mRNA-1273 group. Bottom row, mRNA control group.
(B–D) Percentage of all memory B cells that are specific for WA1 S-2P (B), B.1.617.2 S-2P (C), or both (D) at weeks 6, 25, and 42 post-immunization and days 7 and 28 post-challenge. Circles in (B)–(D) indicate individual NHPs. Boxes represent interquartile range with the median denoted by a horizontal line. 4–7 NHPs per group. Break in y axis indicates a change in scale without a break in the range depicted.
(E) Pie charts indicating the proportion of SARS-CoV-2 S-specific memory B cells that bind to WA1 S-2P alone (light gray), B.1.617.2 S-2P alone (black), or both (dark gray) at weeks 6, 25, and 42 post-immunization and day 28 post-challenge. Values inside pie charts represent the percentage of all memory B cells that bind any SARS-CoV-2 S-2P probe at each time point. 4–7 NHPs per group. Top row, mRNA-1273 group. Bottom row, mRNA control group.
(F) Pie charts indicating the proportion of SARS-CoV-2 S-specific B cells with a phenotype consistent with naive (white), tissue-like memory (light gray), resting memory (dark gray), or activated memory (black) cells at weeks 6, 25, and 42 post-immunization and day 28 post-challenge. 4–7 NHPs per group. Top row, mRNA-1273 group. Bottom row, mRNA control group.