Table 2.
Primer sequences of qPCR.
| Gene name | Primer sequence (5′ to 3′) | Accession number |
|---|---|---|
| Occludin | F:ACGGCAGCACCTACCTCAA | NM_20512.81 |
| R:GGGCGAAGAAGCAGATGAG | ||
| Claudin-1 | F:CATACTCCTGGGTCTGGTTGGT | AY750897.1 |
| R:GACAGCCATCCGCATCTTCT | ||
| ZO-1 | F:CTTCAGGTGTTTCTCTTCCTCCTC | XM_413773 |
| R:CTGTGGTTTCATGGCTGGATC | ||
| TLR-4 | F:GGATCTTTCAAGGTGCCACA | AY064697 |
| R:CAAGTGTCCGATGGGTAGGT | ||
| IL-1β | F: ACTGGGCATCAAGGGCTA | NM_204524.1 |
| R: GGTAGAAGATGAAGCGGGTC | ||
| TNF-α | F:GAGCGTTGACTTGGCTGTC | XM_204267 |
| R:AAGCAACAACCAGCTATGCAC | ||
| IFN-γ | F: AGCTGACGGTGGACCTATTATT | NM_205149.1 |
| R: GGCTTTGCGCTGGATTC | ||
| IL-4 | F:GCTCTCAGTGCCGCTGATG | NM-0010079.1 |
| R:GAAACCTCTCCCTGGATGTCAT | ||
| Mucin-2 | F:TTCATGATGCCTGCTCTTGTG | XM_421035 |
| R:CCTGAGCCTTGGTACATTCTTGT | ||
| GADPH | F:TGCTGCCCAGAACATCATCC | NM_204305.1 |
| R: ACGGCAGGTCAGGTCAACAA |
F, forward; R, reverse; Primers were synthesized by Biotech (Shanghai) Co, Ltd.