Table 7.
The mRNA expressions of tight junctions and inflammatory cytokine in the jejunum of AA broilers on d 21.
| Items | Group |
SEM | Main effect |
P value |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CON | GOD | Cp | Cp + GOD | Infection |
GOD |
Infection | GOD | Infection × GOD | ||||
| - | + | - | + | |||||||||
| Occludin | 1.00 | 1.30 | 1.18 | 1.39 | 0.058 | 1.14 | 1.28 | 1.09b | 1.35a | 0.212 | 0.026 | 0.642 |
| Claudin-1 | 1.00 | 1.45 | 1.20 | 1.46 | 0.094 | 1.22 | 1.33 | 1.10 | 1.46 | 0.564 | 0.065 | 0.617 |
| ZO-1 | 1.00 | 1.47 | 1.08 | 1.60 | 0.079 | 1.24 | 1.34 | 1.04b | 1.53a | 0.445 | 0.001 | 0.868 |
| IL-1β | 1.00 | 1.65 | 1.19 | 1.13 | 0.127 | 1.32 | 1.16 | 1.09 | 1.39 | 0.519 | 0.246 | 0.174 |
| IL-4 | 1.00 | 0.96 | 0.80 | 0.91 | 0.039 | 0.98 | 0.86 | 0.90 | 0.94 | 0.118 | 0.624 | 0.326 |
| Mucin-2 | 1.00 | 0.74 | 0.92 | 0.86 | 0.046 | 0.87 | 0.89 | 0.96 | 0.80 | 0.800 | 0.085 | 0.266 |
| IFN-γ | 1.00 | 1.45 | 1.66 | 1.74 | 0.115 | 1.23b | 1.70a | 1.33 | 1.59 | 0.037 | 0.230 | 0.392 |
| TNF-α | 1.00 | 1.15 | 1.06 | 1.16 | 0.041 | 1.07 | 1.11 | 1.03 | 1.15 | 0.693 | 0.144 | 0.719 |
Abbreviations: Cp, Clostridium perfringens; CON, The control group; GOD, glucose oxidase; GOD, The GOD group; Cp, The Cp challenge group; Cp + GOD, The Cp challenge group fed with GOD.
a–b
Means in the same row without the same superscript differ significantly (P < 0.05). SEM means standard error of the mean. “+” means challenge or add. (n = 7).