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Factors for optimizing extraction method of sample pretreatment.
| Level | Factor | ||
|---|---|---|---|
| Extraction solvent | Extraction method | Extraction time (min) | |
| 1 | 80%Acetonitrile (0.1%FA) | Shock | 10 |
| 2 | 80%Acetonitrile (0.1%HAc) | Ultrasound | 15 |
| 3 | Methanol:Acetonitrile:H2O(2:2:1) | Stand still | 30 |
