(A-C) Immunostains of Somatostatin within the lower cortical layers of the frontal lobe. (D) Quantification of the average percentage of Casp3+NeuN+ within the piglet frontal lobe. (E) Quantification of the average density of SST+ cells. (F-H) Immunostains of Parvalbumin within the lower cortical layers of the frontal lobe. (I) Quantification of the average density of PV+ cells. n = 3 to 4 animals per group. Two-way ANOVA with Tukey’s post-hoc test. Scale bars, 50 μm. Casp3, cleaved-caspase 3; NeuN, neuronal nuclear protein; PV, parvalbumin; SST, somatostatin.