Figure 7.

CCAT2 activated the AKT/GSK3β signaling pathway, promoted cell cycle and EMT, while these effects were rescued by RAB14. (A, B) Phosphorylation levels of AKT/GSK3β in response to ectopic CCAT2 in SW620 and LOVO cells. The Relative fold change in phosphorylation was calculated by the ratio of the phosphorylation signal over total protein signal of AKT and GSK3β. Simultaneously, the expressions of cell cycle and EMT-related factors were also quantified. The endogenous levels of GAPDH protein were used for normalization. The data are represented as mean ± SD. Three independent biological repeats were used for each analysis. *P < 0.05, **P < 0.01.