Fig. 6. PPARα ablation reduces LD size and amount, and suppresses PLIN2 in small intestine.

a Neutral lipids in cytosolic LDs in proximal jejunum stained green by LipidTOX, one hour after 100 μl olive oil gavage. b Representative Oil red O staining of jejunum cryosection 1 h after olive oil gavage. c–e Cytosolic LD area as a percentage of total epithelial area (c), lipid droplet size distribution (d) and contribution of different cytosolic LD sizes toward total lipid area (e), quantified from Oil red O stainings of proximal jejuna, 1 h after olive oil load, n = 6 female mice per group (each sample is the average of three sections. f Relative qPCR gene expression, normalized to Tbp, in jejuna of fasted mice on HFD, n = 7 per group. g Relative RNA sequencing gene expression of Plin2 and three in duodenum of Ppara mice on HFD, n = 3 (lox/lox), four (I-KO mice). h Western blot against perilipin-2 in the organoids from Ppara lox/lox or I-KO, treated with 0.4 mM palmitate: BSA, or BSA. i Expression of Ppara target genes in the crypts as in (h), pool of two experiments, relative to lox/lox vehicle group, n = 7 for vehicle groups, 3–4 for treatments. All data represent mean ± S.D, *P ≤ 0.05, **P < 0.01, ***P < 0.001 of unpaired two-sided t-test confidence level 95%. Source data are provided as a Source Data file.