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. 2021 Nov 26;53(11):1769–1780. doi: 10.1038/s12276-021-00701-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Representative low-magnification images (top), high-magnification images of the boxed areas (middle) and 3D reconstruction images (bottom) of MEFs immunostained with antibodies against CTRP1 (red) and DRP1 (green) and the mitochondrial protein COX4 (blue). The white arrows indicate colocalization of CTRP1 and DRP1. Scale bars, 1 µm (top) and 10 µm (bottom). b Whole-cell lysates (WCLs) of 293T cells cotransfected with constructs expressing Flag–CTRP1 and GFP–DRP1 were immunoprecipitated (IP) with anti-Flag beads and then immunoblotted (IB) with a GFP-specific antibody. Asterisks indicate immunoglobulin G (IgG). c Interaction between endogenous DRP1 and Flag-CTRP1; 293T cells were transfected with Flag–vector (Lane 1) or Flag–CTRP1 (Lane 2), and WCLs were immunoprecipitated with anti-Flag beads and blotted with anti-DRP1. d Interaction between endogenous DRP1 and CTRP1. WCLs of 293T cells were immunoprecipitated with anti-CTRP1 and blotted with anti-Drp1.