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. 2021 Nov 26;53(11):1769–1780. doi: 10.1038/s12276-021-00701-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a and e MitoTracker and anti-Drp1 staining of the indicated cells. Quantification of the Drp1 fluorescence signal from three independent biological replicates is shown. b, d and f Immunoblotting of mitochondrial fractions and WCLs with antibodies against DRP1 and the mitochondrial protein Tom20. c Immunoblotting of the Alb–Cre⁻Ctrp1^fl/fl and Alb–Cre⁺Ctrp1^fl/fl cells with antibodies against CTRP1 and the cytosolic marker tubulin. The asterisks in f represent nonspecific bands. Data are presented as the mean and s.e.m. *P < 0.001. Scale bars, 10 μm (a) and (e).