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. 2021 Nov 24;53(11):1759–1768. doi: 10.1038/s12276-021-00707-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a A schematic diagram and list of the putative genes regulated directly by Sox2 T258 O-GlcNAcylation are shown. Genes that produced anti-Sox2 antibody ChIP signals in the gene region (GSE44288)³², those with downregulated expression in the E14-Sox2^TA/WT cells, and those with upregulated expression after streptozotocin (STZ) treatment (GSE36322)³ were considered putative genes activated by Sox2 T258 O-GlcNAcylation. Repressed genes were also defined based on similar logic. b Anti-Sox2 antibody ChIP-seq binding profiles are shown for certain putative genes regulated by Sox2 T258 O-GlcNAcylation. Sox2 occupancy at putative target gene loci in ESCs was visualized using Integrated Genomics Viewer³³. c The expression of some putative target genes was examined in the E14 and E14-Sox2^TA/WT cells using qPCR. The graph presents the relative expression levels (mean ± standard deviation, n = 3) after normalization to 18S rRNA expression. *P < 0.05, **P < 0.01, ***P < 0.001.