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. 2021 Dec 2;12:7036. doi: 10.1038/s41467-021-27280-x

Fig. 4. Hypothesis-free identification of surfaceome signaling interactions in prokaryotic systems.

Fig. 4

a Schematic of coupling the peptidomimetic antibiotic Thanatin to the singlet oxygen generator (SOG) thiorhodamine. b Volcano plot showing relative abundance changes of LUX-MS quantified proteins from Thanatin-SOG treated Escherichia coli illuminated for 5 min with and without Thanatin competition, tested using a two-sided Student’s t test. Blue dots represent significantly enriched proteins and red dots represent the direct binding targets of Thanatin previously found by photo-crosslinking experiments. The main targets and selected surfaceome interactors are highlighted. c Schematic representation outlining the molecular mechanism of action of Thanatin in E. coli cells with spatial context. Source data are provided as a Source Data file and interactive volcano plots (Supplementary Data 4).