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. 2021 Dec 2;11:23304. doi: 10.1038/s41598-021-01826-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) PCoA plot of the Bray Curtis distances of the faecal microbiota of pigs on all five treatments across all sampling time points. (B–F) PCA plots of the intergroup variation for amplicon sequence variant (ASV) representation at the genus level of the faecal microbiota of Con/Con and AB + ZnO treatments during five time points post-weaning (PW): (B) Day 13 PW, (C) Day 27 PW, (D) Day 35 PW, (E) Day 55 PW, and (F) Day 100 PW. Shapes indicate the day PW that pigs were sampled. Colours indicate the treatments as follows (1st stage weaning period/2nd stage weaning period): Con/Con, Con/Pro, Pro/Con, Pro/Pro, and AB + ZnO; where Con = control, Pro = probiotic and AB + ZnO = antibiotic + ZnO.