. 2021 Dec 2;11:23304. doi: 10.1038/s41598-021-01826-x

Table 3.

Effect of Bacillus altitudinis WIT588 spore supplementation to 1st stage and/or 2nd stage weaner diets and antibiotic (AB) + ZnO supplementation to 1st stage weaner diets on faecal counts of B. altitudinis WIT588 (log₁₀ CFU/g)¹ at days 13, 27, 35 and 55 post-weaning.

	Treatment					SEM	P-value
Treatment day 0–28 PW	Con	Con	Pro	Pro	AB + ZnO		P-value
Treatment day 29–56 PW	Con	Pro	Con	Pro	Con		Treatment	Day	Treatment × Day
	(No. detected/No. sampled)	(No. detected/ No. sampled)	(No. detected/No. sampled)	(No. detected/No. sampled)	(No. detected/No. sampled)
N	10	10	10	10	10
Day (PW)
13	3.00^2,a (0/10)	3.00^a (0/7)	5.94^b (10/10)	6.00^b (8/8)	3.00^a (0/10)	0.048	< 0.001³
27	3.00^a (0/10)	3.30^a (2/7)	6.06^b (9/9)	6.06^b (10/10)	3.56^a (2/9)	0.199	< 0.001³
35	3.00^a (0/10)	6.00^c (10/10)	3.45^b (9/10)	5.83^c (10/10)	3.00^a (0/10)	0.065	< 0.001³
55	3.14^a (1/10)	5.94^b (10/10)	3.04^a (2/10)	6.15^b (10/10)	3.00^a (0/10)	0.096	< 0.001³
						0.059	< 0.001	0.05	< 0.001

PW, post-weaning; Con, control; Pro, probiotic; AB + ZnO, antibiotic + zinc oxide.

¹Least square means and pooled standard errors of the mean (SEM).

²The limit of detection of the assay for B. altitudinis WIT588 was 1000 CFU/g faeces. Values below the limit of detection were recorded as 3.00 log₁₀ CFU/g faeces.

³P-values represent simple main effects obtained using the Slice option.

^a-cValues within a row that do not share a common superscript are significantly different (P ≤ 0.05).

Data were analysed as repeated measures using the mixed model procedure in SAS with the pig being the experimental unit. Differences in least square means were explored using the t-test after Tukey adjustment for multiple comparisons. The Slice option was used to obtain Simple Main Effects. P values which are less than or equal to 0.05, and therefore denote significant differences, are shown in bold.