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. 2021 Nov 29;53(11):1781–1791. doi: 10.1038/s12276-021-00708-6

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© The Author(s) 2021

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Fig. 6 — a Alizarin red S staining after MSX1 and MSX2 overexpression and Pi treatment. b Measurement of the calcium content. c Alizarin red S staining after MSX1 and MSX2 knockdown and Pi treatment. d Measurement of the calcium content. e Western blot analysis. f Chromatin immunoprecipitation analysis. Effects of Msx2 knockdown on Msx1 (left panel) and vice versa (right panel). g Calcium content. Effects of Msx2 knockdown on Msx1 (left panel) and vice versa (right panel). A10 cells were used.