FIG. 8.

Effects of smf null mutations. (A) The indicated strains were grown in MDM supplemented with 150 nM CuSO₄ and 10 mM ZnCl₂ (the medium was depleted of iron and manganese to maximize expression of the Smf proteins). Cells were washed and prepared for atomic absorption spectroscopy analysis of manganese content. The following strains were analyzed: wild type (Wt), YR98; smf2Δ, XL117; smf3Δ, MP112; smf1Δ smf2Δ, XL131; and smf1Δ smf2Δ smf3Δ, MP113. (B) The indicated strains were tested for expression of an FET3-lacZ reporter plasmid as described in Materials and Methods. Beta-galactosidase activity is reported as units of absorption at 420 nm per microgram of lysate protein. Strains utilized are as described in the legend to panel A.