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. 2021 Dec 2;12(12):1124. doi: 10.1038/s41419-021-04416-x

Fig. 1. Expression of FUT8 is reduced in osteosarcoma tissue and osteosarcoma cell lines.

Fig. 1

A Relative expression of FUT8 mRNA in human osteosarcoma (OS) tissue samples (N = 15) and adjacent tumor-free cancellous bone tissue samples (N = 5). Individual qRT-PCR values of each sample are superimposed on the group mean and SD bars. B Mean relative ±SD (N = 3) expression of FUT8 mRNA in OS cell lines (MNNG, U2OS, 143B) and the osteoblast cell line hFOB1.19. C Western blot of FUT8 protein (upper panels) and quantitation of protein levels (lower bar graphs) in OS cell lines and hFOB1.19 cell extracts (N = 3). D Representative images of FUT8 immunohistochemical staining in OS tissue sections and adjacent tumor-free bone marrow tissue sections. Immunostaining was visualized with DAB with peroxidase as the chromogen. Tissue sections are counterstained with hematoxylin (D scale bars, 100 μm). *P < 0.05; **P < 0.01; ***P < 0.001.