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. 2021 Dec 2;12(12):1124. doi: 10.1038/s41419-021-04416-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A, B Volcano plots show the differentially expressed genes (DEGs) between the two FUT8-regulated cells and their controls. Fold-change values on the abscissa were log2-transformed, and P values on the ordinate were -log10 transformed. The selection criteria of differential expressed genes (DEG) was P-value < 0.05 and changed expression higher than 1.5 or lower than 0.67 fold. C, D KEGG pathway enrichment analysis for DEGs between the two FUT8-regulated cells and their controls. E Mean ± SD (N = 3) relative expression of DEGs. Among the DEGs, genes that are highly related to the TNF/NF-κB2 pathway were verified by RT-qPCR to be expressed in the FUT8-altered cells and their controls. ns På 0.05; *P < 0.05; **P < 0.01; ***P < 0.001.