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. 2021 Dec 2;12(12):1124. doi: 10.1038/s41419-021-04416-x

Fig. 7. Lack of core fucosylation of TNFR shifts TNF signaling.

Fig. 7

A Diagram illustrating the method for detecting core-fucosylation level of TNFR in FUT8 regulated cells and their according control cells. TNFR was first enriched from the four cell strains by immunoprecipitation using an anti-TNFR antibody. Then, Western blot and lectin blot, respectively, were used to detect expression of TNFR and core-fucosylation level of TNFR. The lectin blot was done using the AAL, which specifically identifies a combination of core-fucosylated structures. Relative expression of core fucosylation was normalized to TNFR expression. B Western blot of TNFR and lectin blot of core fucosylation of TNFR. Core fucosylation per unit TNFR was increased in MNNG-F8 but reduced in MNNG-siF8 cells compared to control cells. C Quantitation of core-fucosylation levels (N = 3). D Western blot analysis of the shifts TNF signaling. The TNFR down-stream adaptor molecules that mediates cell apoptotic FADD and TRADD were detected by Western blot. The expression of FADD was obvious changed after altering FUT8 levels, while that of TRADD did not change. Relative expression of proteins was normalized to GAPDH. *P < 0.05.