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. 2021 Dec 2;11:23285. doi: 10.1038/s41598-021-02683-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibition of FAK phosphorylation differentially affects VSMC spheroid formation and morphology. Human VSMCs treated with FAK inhibitor (PF573228) or DMSO (vehicle control) in high-glucose DMEM containing 10% FBS were incubated for 24 h to generate human VSMC spheroids. Total cell lysates were immunoblotted (A) for phosphorylated FAK at Tyr397 (pFAK) and GAPDH. The bar graph displays the pFAK levels normalized to the DMSO control (B). (C) Cultures were imaged using an upright microscope. n = 3 (A–B) and n = 12 (C) biological replicates were used. ***p < 0.001.