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. 2021 Nov 19;9:760309. doi: 10.3389/fbioe.2021.760309

FIGURE 5.

FIGURE 5

Human iPSC-ECFCs start to gain native EC phenotype in repopulated lungs. (A) Immunostaining of CD31 and Laminin in iPSC-ECFC-repopulated lungs. Scale bar 2,000 μm. (B–D) H/E image of a representative section iPSC-ECFC repopulated lungs. Arrows in (B) point to reconstituted capillary lumens; arrows in (C) point to endothelial cells in the vasculature but not in bronchia. V: Blood Vessel; Br: Bronchi (E) Immunostaining for CD31, laminin, ZO1, eNOS, and vWF in repopulated lungs, scale bar 20 μm. (F) Venous outflows versus time, n = 3. (G) Gene ontology analysis of the top DEGs (p < 0.05) enriched in iPSC-ECFC lungs as compared to pre-seeded iPSC-ECFCs. (H) Dot plots show the comparison for top 10 select native markers of human pulmonary venous, arterial, gCap, and aCap amongst native human lung endothelium, pre-seeded iPSC-ECFCs, and iPSC-ECFC-repopulated lungs. Samples were merged and re-scaled.