Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Nov 19;9:758402. doi: 10.3389/fcell.2021.758402

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 De Rosa, Johnson and Opresko.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

The FAP-mCer-TRF1 system. The targeting tool that specifically generates 8-oxoG at telomeres, consists of an overexpressed shelterin protein TRF1 fused with the fluorogen-activating peptide (FAP), along with the fluorescent protein m-Cerulean (mCer) to visualize expression. FAP-mCer-TRF1 expressing cells are preincubated with 100 nM of the photosensitizer dye di-iodinated malachite green (MG2I), which when bound to the FAP, produces singlet oxygen (¹O₂) upon excitation with 660 nm light. The singlet oxygen then reacts specifically with the telomeric guanines to generate 8-oxoG in a temporally and spatially controlled manner.