Table 1.

List of antibodies and viability dye used in 31-color spectral flow cytometry panel.

No.	Excitation laser	Fluorochrome	Marker	Clone	Manufacturer	Catalog no.	mAb mix	Reference controls
1	355nm/UV	BUV395	CD8	RPA-T8	BD	563795	Surface 1	Cells
2		Zombie UV	Viability		BioLegend	423108	Viability	Cells
3		BUV496	CD4	RPA-T4	BD	741134	Surface 1	Cells
4		BUV563	CD161	HP-3G10	BD	749223	Surface 1	Beads
5		BUV615	CD14	M5E2	BD	751150	Surface 1	Cells
6		BUV661	CD69	FN50	BD	750213	Surface 1	Beads
7		BUV737	TCRgd	11F2	BD	748533	Surface 1	Cells
8		BUV805	CD56	NCAM162	BD	749086	Surface 1	Cells
9	405nm/V	BV421	CCR6	G034E3	BioLegend	353408	Surface 1	Cells
10		BV480	CCR4	1G1	BD	746361	Surface 1	Beads
11		BV510	CD45RA	HI100	BioLegend	304142	Surface 1	Cells
12		BV570	HLA-Dr	L243	BioLegend	307638	Surface 1	Beads
13		BV605	Ki-67	Ki-67	BioLegend	350522	Intracellular	Beads
14		BV650	CXCR3	G025H7	BioLegend	353730	Surface 1	Beads
15		BV711	CD45RO	UCHL1	BioLegend	304236	Surface 1	Cells
16		BV750	CXCR5	J252D4	BioLegend	356942	Surface 1	Beads
17		BV785	CCR7	G043H7	BioLegend	353230	Surface 1	Cells
18	488nm/B	BB515	CD25	2A3	BD	564467	Surface 1	Cells
19		Spark Blue	CD3	SK7	BioLegend	344852	Surface 2	Cells
20		PerCP	CD19	HIB19	BioLegend	302228	Surface 2	Cells
21		BB700	CD49b	AK-7	BD	746009	Surface 1	Beads
22		PerCP-eF710	CD127	EBioRDR5	eBioscience	46-1278-42	Surface 2	Cells
23	561nm/YG	PE	FOXP3	PCH101	eBioscience	12-4776.42	Intracellular	Beads
24		PE-Dazzle594	TIGIT	A15153G	BioLegend	372715	Surface 2	Beads
25		PE-Cy5	GITR	621	BioLegend	311608	Intracellular	Beads
26		PE-Cy7	PD-1	EH12.2H7	BioLegend	329918	Surface 2	Beads
27	640nm/R	APC	CCR10	314305	R&D	FAB3478A	Surface 2	Cells
28		eFluor660	CTLA4	14D3	eBioscience	50-1529-49	Intracellular	Beads
29		APC-R700	LAG-3	T47-530	BD	565774	Surface 2	Beads
30		APC-Fire750	ICOS	C398.4A	BioLegend	313536	Surface 2	Beads
31		APC-Fire810	CD27	QA17A18	BioLegend	393214	Surface 2	Cells