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. 2021 Nov 19;9:771066. doi: 10.3389/fbioe.2021.771066

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Copyright © 2021 Huang, Luo, Zhang, Ying, Cai, Lu, Zhao, Wang, Lin, Tu, Xiang, Wu, Yang, Zhu and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Application of NT results in a better histologic state of the flap. (A) H and E staining images of the control, NT, and NT+3MA groups. Magnification was × 10, Scale bar = 200 μm. Area selected by the white border is taken at high magnification as the representative image, Scale bar = 50 μm. (B) Number of new blood vessels. (C) Masson’s trichrome stain images of each group showing blood vessels and collagen fibers. Scale bar = 200 μm. High-power images were also equipped, Scale bar = 50 μm. (D) Percentage of the tissue water content of the flap on day 7. Data were expressed as mean ± SD, n = 3. “*” and “**” represent p <0.05 or p <0.01 versus the NT group.