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. 2021 Nov 19;9:771066. doi: 10.3389/fbioe.2021.771066

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Copyright © 2021 Huang, Luo, Zhang, Ying, Cai, Lu, Zhao, Wang, Lin, Tu, Xiang, Wu, Yang, Zhu and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NT improves autophagy levels (A) Western blot results showing the expression of Beclin-1, VPS34, LC3, and P62 in different groups. (B–E) Quantitative analysis of Beclin-1, VPS34, LC3, and P62 protein expressions. (F) Immunofluorescence showing the expression of LC3B (red), P62 (green), and DAPI (blue) in different groups. Magnification: 20X; Scale: 100 μm. (G–H) Mean fluorescence intensity of LC3B and P62 in different groups. Data were expressed as mean ± SD, n = 3. “**” and “*” represent p <0.01 or p <0.05 versus the NT group, indicating statistical significance.