TABLE 3.

A summary of the most applicable tools to design sgRNA.

Tool	Website	Species support	Nucleases	Nickase	Batch mode	Off-target analysis	CRISPR/Cas system
ZiFiT Sander et al. (2007)	http://zifit.partners.org/ZiFiT	9	Yes	Yes	No	Yes	Type II only
CRISPR direct Naito et al. (2015)	http://crispr.dbcls.jp	18	Yes	No	No	Yes	Type II only
CHOPCHOP Labun et al. (2016)	https://chopchop.rc.fas, harvard.edu	25	Yes	No	No	Yes	Different Type II
CRISPR design Hsu et al. (2013)	http://crispr.mit.edu	16	Yes	Yes	Yes	Yes	Type II only
E-CRISPR Heigwer et al. (2014)	http://www.e-crisp.org/E-CRISP	33	Yes	Yes	No	Yes	Different Type II
CRISPR RGEN tools Bae et al. (2014)	http://www.rgenome.net	16	Yes	No	No	Yes	Different Type II
CRISPR MultiTargeter Prykhozhij et al. (2015)	http://www.multicrispr.net	12	Yes	Yes	Yes	No	Multiple types
sgRNA Designer Kim et al. (2014)	http://broadinstitute.org/rnai/public/analysis-tools/sgrna design	2	Yes	No	Yes	No	Type II only
CRISPRscan Moreno-Mateos et al. (2015)	http://crisprscan.org	7	Yes	No	No	Yes	Type II only
CRISPR-ERA Liu et al. (2015)	http://crisprera.stanford.edu/InitAction.action	9	Yes	Yes	No	Yes	Type II only
sgRNA Scorer Chari et al. (2017)	https://crispr.med.harvard.edu/sgRNAScorer	12	Yes	No	No	No	Different Type II