Figure 6.
Correlation analysis of transcriptome and proteome data from O. longistaminata anthers of different spikelet growth stages. (A) Protein and transcript GEST analysis. Pathways with NOM p<0.05 were used to draw a GSEA analysis chart. Correlation is close to 1.0, indicating a positive correlation between gene translation and protein translation. Correlation is close to −1.0, indicating a negative correlation. The reliability of Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment results was high when NES was close to 2, and low when NES was close to −2. (B) Cluster analysis of combined protein and transcriptome data. Expression values were log2-transformed, and the mean value was subtracted; then the Hclust “Ward.D” method was used for cluster analysis. (C) KEGG enrichment analysis was performed for each protein class by Fisher’s exact test method, and the enrichment results for different classifications were combined. Then, the top 40 (p<0.05) significantly enriched functional classifications were identified. The horizontal axis shows fold enrichment after Log2 transformation, the vertical axis shows the functional classification, the bubble size represents the number of proteins, and the bubble color represents the value of p of enrichment significance.