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. 2021 Nov 19;9:784244. doi: 10.3389/fcell.2021.784244

FIGURE 4.

FIGURE 4

Vitamin C enhances TET enzymatic activity in IVF preimplantation embryos (A, B) Effects of supplementation with vitamin C or α-KG alone, or in combination on active DNA demethylation in IVF zygotes (A) and blastocysts (B). Left panels in A and B: Immunofluorescent images of 5mC (green) and 5hmC (red) staining in embryos in each group. Right panels in A and B: quantification of 5hmC/5mC ratio in IVF embryos in the control group or treatment groups. Circles represent the relative ratio in each embryo. The number of embryos in each group is indicated (C) Effects of vitamin C supplementation on active DNA demethylation in bovine IVF preimplantation embryos. Lower panels in C: quantification of 5hmC/5mC ratio in bovine IVF embryos in the control group or treatment group. Circles represent the relative ratio in each embryo. The number of embryos in each group is indicated. Data shows the means ± SD of three independent experiments. *p < 0.05, **p < 0.01. Scale bar, 50 μm.