Skip to main content
. 2021 Nov 19;9:784244. doi: 10.3389/fcell.2021.784244

FIGURE 6.

FIGURE 6

The beneficial effects of vitamin C on IVF embryos are mediated by TET enzymatic activity (A, B) Effect of supplementation with vitamin C or TET inhibitor DMOG alone, or in combination on active DNA demethylation in IVF zygotes (A) and blastocysts (B). Left panels in A and B: Immunofluorescent images of 5mC (green) and 5hmC (red) staining in embryos in each group. Right panels in A and B: quantification of 5hmC/5mC ratio in IVF embryos in the control group or treatment groups. Circles represent the relative ratio in each embryo. The number of embryos in each group is indicated (C) Immunofluorescent images of NANOG and CDX2 in IVF blastocysts incubated with vitamin C or DMOG alone, or in combination. The embryos were counterstained with DAPI (D-F) Effect of supplementation with vitamin C or DMOG alone, or in combination on total cell numbers (D), ICM cell numbers (E), and ratios of ICM/TE (F) in IVF blastocytes. Data show the means ± SD of three independent experiments. *p < 0.05, **p < 0.01. Scale bar, 50 μm.