FIGURE 4.

PPARα antagonist attenuates the effects of (+)-dehydrovomifoliol on OA-induced lipid accumulation in HepG2 cells. Before induction with oleic acid (OA) for 24 h, HepG2 cells were pretreated with 10 μM GW6471 for 2 h and with 10 μM compound 1 for 30 min. (A) Oil red O staining images (200×) and (B) the percentages of HepG2 cells containing lipid droplets. (C) Triglyceride (TG) levels were analyzed using the TG detection assay. (D, E, F) Western blotting analysis of SREBP1 and FGF21 protein levels in HepG2 cells and quantification by densitometric scanning. (G), (H) mRNA levels of ACC, FASN, SREBP1, ACOX1, and FGF21 were detected using RT-QPCR. Data shown in the bar chart are expressed as the mean ± SEM (n = 3). ^* p < 0.05, ^** p < 0.01, ^**** p < 0.0001.