FIGURE 2.

Effects of HIF-silencing on Arg-II expression under hypoxia conditions in HK-2 cells. HK-2 cells were transduced with rAd/U6-LacZ^shRNA as control or rAd/U6-hif1α^shRNA and rAd/U6-hif2α^shRNA to silence hif1α and hif2α gene, respectively. Immunoblotting analyses illustrating effects of (A) hif1α silencing, (B) hif2α silencing, and (C) simultaneous silencing of both hif1α and hif2α on Arg-II upregulation in HK-2 cells under hypoxia conditions. The graphs on the right indicate the quantification of the signals on the immunoblots. Data are presented as mean ± SD. *P < 0.05, ****P < 0.0001 between the indicated groups. ANOVA with Bonferroni post hoc test was performed.