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. 2021 Nov 27;12:100173. doi: 10.1016/j.mtbio.2021.100173

Fig. 2.

Fig. 2

Calcium deposition in the bone layer scaffold and intermediate layer scaffold. A) Calcium deposition in the bone layer scaffold in osteogenic media. B) Calcium deposition in the bone layer scaffold in standard growth media. C) Alizarin red staining of the bone layer scaffold in osteogenic and standard media (Scale ​= ​1000 ​μm). D) Calcium deposition in intermediate layer scaffold. (∗∗∗p ​≤ ​0.001, ∗p ​≤ ​0.05).