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Chondrogenesis in the cartilage layer scaffold. A) Quantification of sGAG in the cartilage layer in chondrogenic media. B) Quantification of sGAG in the cartilage layer in standard growth media. C) Toluidine Blue staining of cartilage layer scaffold cultured in chondrogenic media and standard media (Scale = 1000 μm). D) Collagen II immunohistochemical staining of cartilage layer scaffold (Scale = 500 μm). (∗∗∗∗p ≤ 0.0001, ∗∗∗p ≤ 0.001, ∗∗p ≤ 0.01, ∗p ≤ 0.05).
