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Safranin-O staining of scaffolds seeded with each cell group at Day 14, Day 21 and Day 28. A) Low density FPMSCs (FPMSCs LD) B) High density FPMSCs (FPMSCs HD), C) Low density CCs (CC LD), D) High density CCs (CC HD) and E) Co-culture of FPMSCs and CCs (FPMSCs:CC). Higher levels of sGAG deposition were observed in the co-culture group (E) at day 21 and the high-density groups (B, D and E) at day 28 (black arrows). Scale = 500 μm.
